Human Anti-Cardiolipin IgM (Cardiolipin IgM) ELISA Kit | KTE63020

Human Anti-Cardiolipin IgM (Cardiolipin IgM) ELISA Kit | KTE63020 | Gentaur UK, US & Europe Distribution

Application: Human Anti-Cardiolipin IgM (Cardiolipin IgM) ELISA Kit quantitate Human Cardiolipin IgM in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. HRP-conjugated anti-human IgM antibody is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

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Calibration Range: 3 U/mL-48 U/mL

Limit Of Detection: 0.1 U/mL

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Tag: Cardiolipin IgM