Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit | KTE62457

Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit | KTE62457 | Gentaur UK, US & Europe Distribution

Application: This Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate Anti-FSH-Ab in samples. An antibody specific for Anti-FSH-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAnti-FSH-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Anti-FSH-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-FSH-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Anti-Follicle-Stimulating Hormone Antibody microplate
• Human Anti-Follicle-Stimulating Hormone Antibody standard
• Human Anti-Follicle-Stimulating Hormone Antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human Anti-FSH-Ab. No significant cross-reactivity or interference between Human Anti-FSH-Ab and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In the ovary FSH stimulates the growth of immature Graafian follicles to maturation. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure; therefore the biological and immunological properties of each hormone are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells; follicular steroidogenesis is promoted and LH production is stimulated.

Alternative Names: Anti-FSH-Ab

Search name: Anti-FSH-Ab

Tag: Anti-FSH