Human AP13 (Apelin 13) ELISA Kit | ELK2252

Human AP13 (Apelin 13) ELISA Kit | ELK2252 | Gentaur UK, US & Europe Distribution

Alternative Names: APLN13

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.24 pg/mL

Standard: 1600 pg/mL

Detection Range: 25-1600 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 13 (AP13) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 13 (AP13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin 13 (AP13) in the samples is then determined by comparing the OD of the samples to the standard curve.