Human AP2m1 (Adaptor Related Protein Complex 2 Mu 1) ELISA Kit | ELK4489

Human AP2m1 (Adaptor Related Protein Complex 2 Mu 1) ELISA Kit | ELK4489 | Gentaur UK, US & Europe Distribution

Alternative Names: AP50; CLAPM1; Clathrin-Associated/Assembly/Adaptor Protein, Medium 1; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Coat Adaptor Protein AP50

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1 (AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1 (AP2m1) in the samples is then determined by comparing the OD of the samples to the standard curve.