Human AP36 (Apelin 36) ELISA Kit | ELK2243

Human AP36 (Apelin 36) ELISA Kit | ELK2243 | Gentaur UK, US & Europe Distribution

Alternative Names: APLN36

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.52 pg/mL

Standard: 600 pg/mL

Detection Range: 9.38-600 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 36 (AP36) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 36 (AP36) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin 36 (AP36) in the samples is then determined by comparing the OD of the samples to the standard curve.