Human AP3b1 (Adaptor Related Protein Complex 3 Beta 1) ELISA Kit | ELK5267

Human AP3b1 (Adaptor Related Protein Complex 3 Beta 1) ELISA Kit | ELK5267 | Gentaur UK, US & Europe Distribution

Alternative Names: HPS; PE; HPS2; ADTB3; ADTB3A; Clathrin assembly protein complex 3 beta-1 large chain; Adaptor protein complex AP-3 subunit beta-1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 3 Beta 1 (AP3b1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 3 Beta 1 (AP3b1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 3 Beta 1 (AP3b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 3 Beta 1 (AP3b1) in the samples is then determined by comparing the OD of the samples to the standard curve.