Human APC (Adenomatous polyposis coli) ELISA Kit | ELK9156

Human APC (Adenomatous polyposis coli) ELISA Kit | ELK9156 | Gentaur UK, US & Europe Distribution

Alternative Names: APC; DP2; DP3; FPC; GS; DP2.5; Protein APC; Min; adenomatosis polyposis coli; adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli; adenomatous polyposis coli protein; BTPS2; Deleted in polyposis 2.5

Species: Human

Assay Type: Sandwich

Sensitivity: 5.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cell Biology; Neuroscience; Epigenetics and Nuclear Signaling

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenomatous polyposis coli (APC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adenomatous polyposis coli (APC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenomatous polyposis coli (APC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenomatous polyposis coli (APC) in the samples is then determined by comparing the OD of the samples to the standard curve.