Human APLN (Apelin) ELISA Kit | ELK4069

Human APLN (Apelin) ELISA Kit | ELK4069 | Gentaur UK, US & Europe Distribution

Alternative Names: XNPEP2; APEL; APJ endogenous ligand

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.25 pg/mL

Standard: 1600 pg/mL

Detection Range: 25-1600 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin (APLN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin (APLN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin (APLN) in the samples is then determined by comparing the OD of the samples to the standard curve.