Human APOA5 (Apolipoprotein A5) ELISA Kit | ELK2741

Human APOA5 (Apolipoprotein A5) ELISA Kit | ELK2741 | Gentaur UK, US & Europe Distribution

Alternative Names: Apo-A5; APOA-V; APOAV; RAP3; Regeneration-associated protein 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.48 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the OD of the samples to the standard curve.