Human APOH (Apolipoprotein H) ELISA Kit | ELK2348

Human APOH (Apolipoprotein H) ELISA Kit | ELK2348 | Gentaur UK, US & Europe Distribution

Alternative Names: Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta (2) GPI

Species: Human

Assay Type: Sandwich

Sensitivity: 0.141 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein H (APOH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein H (APOH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein H (APOH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein H (APOH) in the samples is then determined by comparing the OD of the samples to the standard curve.