552

Human Apolipoprotein AII (Apo AII) | ABMC-P05

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SKU:
552-P05-GEN
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Description

Human Apolipoprotein AII (Apo AII) |P05

Concentration: 1 mg / ml, determined by the Lowry method 
Source: From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Purification: After series ultracentrifugations, High Density Lipoprotein (HDL) is isolated from human plasma. Apo AII is purified from delipidated HDL, followed by gel-filtration and DEAE-Sepharcyl chromatography.
Purity: ≥ 98% by SDS-PAGE
Buffer: 20 mM Tris-HCl, 140 mM NaCl, 0.02% NaN3, 0.5 mM EDTA, pH 7.4.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

IMPORTANCE

Apo AII comprises 25% of HDL. It exists in human plasma as a dimer of 2 identical chains of 77 amino acid residues, joined by disulfide. The molecular weight is reported to be 8.7 kDa for a single chain (Brewer et al., 1972).

Studies on mouse reported that apo AII may be proatherogenic (Warden et al., 1993); however, case-control study in the large European Prospective Investigation demonstrated that plasma Apo AII concentrations were strongly inversely correlated with CHD events (Birjmohun et al., 2007).

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