552

Human Apolipoprotein E (Apo E) | ABMC-P16

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SKU:
552-P16-GEN
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Description

Human Apolipoprotein E (Apo E) |P16

 

Concentration: 0.9 mg / ml, determined by the Lowry method
Source: From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Purification: After series ultracentrifugations, Very Low density Lipoprotein (VLDL) is isolated from human plasma. Apo E is purified from delipidated VLDL, followed by gel-filtration. Minor impurities of other apo-proteins are then removed by antibody-Sepharose™ affinity column.
Purity: ≥ 98% by SDS-PAGE
Buffer: 20 mM Tris-HCl, 0.15 mM NaCl, 0.5 mM EDTA, 0.02 % NaN3, pH 7.4.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

IMPORTANCE

Apo E contains 299 amino acid residues. It is a 34-37 kDa glycosylated protein (Rall et al., 1983).

Apo E is involved with triglyceride, phospholipid, cholesteryl ester, and cholesterol transport in and out of cells and is a ligand for LDL receptors. Apo E has also been implicated in immune and nerve degeneration. It has been found to suppress lymphocyte proliferation. Late-onset familial and sporadic Alzheimer disease patients have been found to have a higher occurrence of one of the three common Apo E isoforms, Apo E4. The Apo E4 isoform has been detected in senile plaques and neurofibrillary tangles of Alzheimer disease patients. Apo E4 is associated with rapid chylomicron-remnant clearance and increased total cholesterol levels.

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