Human Apoptosis protease activating factor-1 (APAF1) ELISA Kit | KTE62504

Human Apoptosis protease activating factor-1 (APAF1) ELISA Kit | KTE62504 | Gentaur UK, US & Europe Distribution

Application: This Human Apoptosis protease activating factor-1 (APAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate APAF1 in samples. An antibody specific for APAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Apoptosis protease activating factor-1 microplate
• Human Apoptosis protease activating factor-1 standard
• Human Apoptosis protease activating factor-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Apoptosis protease activating factor-1 (APAF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human APAF1. No significant cross-reactivity or interference between Human APAF1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Apoptotic peptidase activating factor 1 is a proteincontains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. The precise mechanism for this reaction is still debated though work published by Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalysis. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis.Alternative splicing results in several transcript variants encoding different isoforms.

Alternative Names: APAF1; APAF-1; CED4; DKFZp781B1145; apoptotic protease activating factor 1

Search name: APAF1; APAF-1; CED4; DKFZp781B1145; apoptotic protease activating factor 1

Tag: APAF1