Human APP (Amyloid Precursor Protein) ELISA Kit | ELK1735

Human APP (Amyloid Precursor Protein) ELISA Kit | ELK1735 | Gentaur UK, US & Europe Distribution

Alternative Names: AAA; ABeta; ABPP; AD1; APPI; CVAP; PN2; CTFgamma; Peptidase Nexin-II Alzheimer Disease; Peptidase Nexin-II; Amyloid Beta (A4) Precursor Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Precursor Protein (APP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amyloid Precursor Protein (APP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Precursor Protein (APP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amyloid Precursor Protein (APP) in the samples is then determined by comparing the OD of the samples to the standard curve.