Human Aquaporin 3 (AQP3) ELISA Kit | KTE62203

Human Aquaporin 3 (AQP3) ELISA Kit | KTE62203 | Gentaur UK, US & Europe Distribution

Application: This Human Aquaporin 3 (AQP3) ELISA Kit employs a two-site sandwich ELISA to quantitate AQP3 in samples. An antibody specific for AQP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAQP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AQP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Aquaporin 3 microplate
• Human Aquaporin 3 standard
• Human Aquaporin 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Aquaporin 3 (AQP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AQP3. No significant cross-reactivity or interference between Human AQP3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: AQP3 encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of AQP3 results in multiple transcript variants encoding different isoforms.

Alternative Names: AQP3; GIL; Gill blood group; aquaporin 3; aquaporin 3 (GIL blood group)

Search name: AQP3; GIL; Gill blood group; aquaporin 3; aquaporin 3 (GIL blood group)

Tag: AQP3