855

Human AS3MT (Arsenic, 3-Oxidation State Methyltransferase) ELISA Kit | ELK5325

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SKU:
855-ELK5325-GEN
NULL588.00 - NULL704.00

Description

Human AS3MT (Arsenic, 3-Oxidation State Methyltransferase) ELISA Kit | ELK5325 | Gentaur UK, US & Europe Distribution

Alternative Names: CYT19; Arsenite Methyltransferase; Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic (III) methyltransferase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arsenic, 3-Oxidation State Methyltransferase (AS3MT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arsenic, 3-Oxidation State Methyltransferase (AS3MT) in the samples is then determined by comparing the OD of the samples to the standard curve.

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