Human ASAH1 (N-Acylsphingosine Amidohydrolase 1) ELISA Kit | ELK2966

Human ASAH1 (N-Acylsphingosine Amidohydrolase 1) ELISA Kit | ELK2966 | Gentaur UK, US & Europe Distribution

Alternative Names: AC; ASAH; PHP; PHP32; Acid Ceramidase; Acylsphingosine deacylase; Putative 32 kDa heart protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.63 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 1 (ASAH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 1 (ASAH1) in the samples is then determined by comparing the OD of the samples to the standard curve.