Human AST (Aspartate Aminotransferase) ELISA Kit | ELK1966

Human AST (Aspartate Aminotransferase) ELISA Kit | ELK1966 | Gentaur UK, US & Europe Distribution

Alternative Names: cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1, Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1, Soluble

Species: Human

Assay Type: Sandwich

Sensitivity: 0.119U/L

Standard: 20U/L

Detection Range: 0.32-20U/L

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the OD of the samples to the standard curve.