Human ATG16L1 (Autophagy Related Protein 16 Like Protein 1) ELISA Kit | ELK6404

Human ATG16L1 (Autophagy Related Protein 16 Like Protein 1) ELISA Kit | ELK6404 | Gentaur UK, US & Europe Distribution

Alternative Names: WDR30

Species: Human

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 16 Like Protein 1 (ATG16L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 16 Like Protein 1 (ATG16L1) in the samples is then determined by comparing the OD of the samples to the standard curve.