Human ATP1a2 (ATPase, Na+/K+ Transporting Alpha 2 Polypeptide) ELISA Kit | ELK7441

Human ATP1a2 (ATPase, Na+/K+ Transporting Alpha 2 Polypeptide) ELISA Kit | ELK7441 | Gentaur UK, US & Europe Distribution

Alternative Names: FHM2; MHP2; Migraine, Hemiplegic 2; Sodium pump subunit alpha-2; Sodium/potassium-transporting ATPase subunit alpha-2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.103 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) in the samples is then determined by comparing the OD of the samples to the standard curve.