Human b2M (Beta-2-Microglobulin) ELISA Kit | ELK1200

Human b2M (Beta-2-Microglobulin) ELISA Kit | ELK1200 | Gentaur UK, US & Europe Distribution

Alternative Names: Beta 2M; BMG; B2MG

Species: Human

Assay Type: Sandwich

Sensitivity: 0.16 ng/mL

Standard: 25 ng/mL

Detection Range: 0.4-25 ng/mL

Sample Type: serum, plasma, urine and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Kidney biomarker

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the OD of the samples to the standard curve.