Human b3GAT1 (Beta-1, 3-Glucuronyltransferase 1) ELISA Kit | ELK4339

Human b3GAT1 (Beta-1, 3-Glucuronyltransferase 1) ELISA Kit | ELK4339 | Gentaur UK, US & Europe Distribution

Alternative Names: CD57; B3-GAT1; GLCATP; GlcAT-P; GlcUAT-P; HNK-1; LEU7; NK-1; Glucuronosyltransferase P; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Metabolic pathway;Neuro science;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1, 3-Glucuronyltransferase 1 (b3GAT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-1, 3-Glucuronyltransferase 1 (b3GAT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1, 3-Glucuronyltransferase 1 (b3GAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1, 3-Glucuronyltransferase 1 (b3GAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.