855
Human b3GNT3 (Beta-1, 3-N-Acetylglucosaminyltransferase 3) ELISA Kit | ELK5071
- SKU:
- 855-ELK5071-GEN
Description
Human b3GNT3 (Beta-1, 3-N-Acetylglucosaminyltransferase 3) ELISA Kit | ELK5071 | Gentaur UK, US & Europe Distribution
Alternative Names: B3GAL-T8; B3GN-T3; B3GNT-3; TMEM3; beta3Gn-T3; Putative Type II Membrane Protein; Transmembrane Protein 3; Core 1 extending beta-1, 3-N-acetylglucosaminyltransferase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1, 3-N-Acetylglucosaminyltransferase 3 (b3GNT3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-1, 3-N-Acetylglucosaminyltransferase 3 (b3GNT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1, 3-N-Acetylglucosaminyltransferase 3 (b3GNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1, 3-N-Acetylglucosaminyltransferase 3 (b3GNT3) in the samples is then determined by comparing the OD of the samples to the standard curve.