Human b4GALT1 (Beta-1, 4-Galactosyltransferase 1) ELISA Kit | ELK2220

Human b4GALT1 (Beta-1, 4-Galactosyltransferase 1) ELISA Kit | ELK2220 | Gentaur UK, US & Europe Distribution

Alternative Names: GTB; B4-GALT1; GGTB2; GT1; Beta4Gal-T1; Lactose Synthase A Protein; N-Acetyllactosamine Synthase; Nal synthase; beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1, 4-Galactosyltransferase 1 (b4GALT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-1, 4-Galactosyltransferase 1 (b4GALT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1, 4-Galactosyltransferase 1 (b4GALT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1, 4-Galactosyltransferase 1 (b4GALT1) in the samples is then determined by comparing the OD of the samples to the standard curve.