Human BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit | ELK3699

Human BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit | ELK3699 | Gentaur UK, US & Europe Distribution

Alternative Names: BIS; CAIR1; BAG Family Molecular Chaperone Regulator 3; Bcl-2-binding protein Bis; Docking protein CAIR-1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated Athanogene 3 (BAG3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Associated Athanogene 3 (BAG3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated Athanogene 3 (BAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated Athanogene 3 (BAG3) in the samples is then determined by comparing the OD of the samples to the standard curve.