Human BAK1 (Bcl2 Antagonist/Killer 1) ELISA Kit | ELK3955

Human BAK1 (Bcl2 Antagonist/Killer 1) ELISA Kit | ELK3955 | Gentaur UK, US & Europe Distribution

Alternative Names: BAK; BAK-LIKE; BCL2L7; CDN1; Bcl-2 Homologous Antagonist/Killer; Apoptosis regulator BAK; Bcl-2-like protein 7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.239 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1 (BAK1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1 (BAK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1 (BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Antagonist/Killer 1 (BAK1) in the samples is then determined by comparing the OD of the samples to the standard curve.