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Human Bap1 (Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit | ELK8569
- SKU:
- 855-ELK8569-GEN
Description
Human Bap1 (Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit | ELK8569 | Gentaur UK, US & Europe Distribution
Alternative Names: BAP1; BRCA1 associated protein 1; Cerebral protein 6; HUCEP 13; hucep 6; KIAA0272; UCHL2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin carboxyl-terminal hydrolase BAP1 (Bap1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquitin carboxyl-terminal hydrolase BAP1 (Bap1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin carboxyl-terminal hydrolase BAP1 (Bap1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin carboxyl-terminal hydrolase BAP1 (Bap1) in the samples is then determined by comparing the OD of the samples to the standard curve.