Human BCL2L11 (Bcl2 Like Protein 11) ELISA Kit | ELK6362

Human BCL2L11 (Bcl2 Like Protein 11) ELISA Kit | ELK6362 | Gentaur UK, US & Europe Distribution

Alternative Names: BOD; BimL; BimEL; BIM; Bcl2-interacting mediator of cell death

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Like Protein 11 (BCL2L11) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Like Protein 11 (BCL2L11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Like Protein 11 (BCL2L11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Like Protein 11 (BCL2L11) in the samples is then determined by comparing the OD of the samples to the standard curve.