Human Beta cellulin (BTC) ELISA Kit | KTE62563

Human Beta cellulin (BTC) ELISA Kit | KTE62563 | Gentaur UK, US & Europe Distribution

Application: This Human Beta cellulin (BTC) ELISA Kit employs a two-site sandwich ELISA to quantitate BTC in samples. An antibody specific for BTC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBTC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BTC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BTC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Beta cellulin microplate
• Human Beta cellulin standard
• Human Beta cellulin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Beta cellulin (BTC) ELISA Kit has high sensitivity and excellent specificity for detection of Human BTC. No significant cross-reactivity or interference between Human BTC and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Betacellulin (BTC), has been identified in the conditioned medium of cell lines derived from mouse pancreatic beta cell tumors. Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. The carboxyl-terminal domain of betacellulin has 50 percent sequence similarity with that of rat transforming growth factor alpha. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.Watanabe et al. purified soluble forms of human BTC to homogeneity from the conditioned medium of mouse A9 cells transfected with the human BTC precursor cDNA. Three types of soluble BTC, apparently derived from a single gene, were identified and their biologic activities assessed.

Alternative Names: BTC

Search name: BTC

Tag: BTC