Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit | AE62214HU

Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit | AE62214HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: B-MSH

Alternative Name: N/A

Application: ELISA

Range: 37.0-3000 pg/mL

Sensitivity: 15.3 pg/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤8.1%

Recovery: 0, 87

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate B-MSH in samples. An antibody specific for B-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-MSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: beta-MSH: a functional ligand that regulated energy homeostasis via hypothalamic MC4-R. beta-MSH is also capable of activating MC4-R and inhibiting feeding. beta-MSH acts as an endogenous MC4-R agonist and that this melanocortin peptide plays a role in the regulation of feeding and energy balance. Food-restriction significantly increased beta-MSH levels in the VMH, DMH and ARC above freely-fed controls, whilst alpha-MSH concentrations were unchanged. beta-MSH has higher affinity at MC4-R than alpha-MSH;beta-MSH activates GPCR in these sites, which are rich in MC4-R; beta-MSH is present in hypothalamic nuclei that regulate feeding and its concentrations alter with nutritional state. beta-MSH rather than alpha-MSH is the key ligand at the MC4-R populations that regulate feeding, and that inhibition of tonic release of beta-MSH is one mechanism contributing to hunger in under-feeding.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .