Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit | KTE62452

Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit | KTE62452 | Gentaur UK, US & Europe Distribution

Application: This Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit employs a two-site sandwich ELISA to quantitate B-MSH in samples. An antibody specific for B-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-MSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Beta-melanocyte stimulating hormone microplate
• Human Beta-melanocyte stimulating hormone standard
• Human Beta-melanocyte stimulating hormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit has high sensitivity and excellent specificity for detection of Human B-MSH. No significant cross-reactivity or interference between Human B-MSH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: beta-MSH: a functional ligand that regulated energy homeostasis via hypothalamic MC4-R. beta-MSH is also capable of activating MC4-R and inhibiting feeding. beta-MSH acts as an endogenous MC4-R agonist and that this melanocortin peptide plays a role in the regulation of feeding and energy balance. Food-restriction significantly increased beta-MSH levels in the VMH, DMH and ARC above freely-fed controls, whilst alpha-MSH concentrations were unchanged. beta-MSH has higher affinity at MC4-R than alpha-MSH;beta-MSH activates GPCR in these sites, which are rich in MC4-R; beta-MSH is present in hypothalamic nuclei that regulate feeding and its concentrations alter with nutritional state. beta-MSH rather than alpha-MSH is the key ligand at the MC4-R populations that regulate feeding, and that inhibition of tonic release of beta-MSH is one mechanism contributing to hunger in under-feeding.

Alternative Names: B-MSH

Search name: B-MSH

Tag: B-MSH