Human Beta-site APP-Cleaving Enzyme 1 (BACE1) ELISA Kit | AE59290HU

Human Beta-site APP-Cleaving Enzyme 1 (BACE1) ELISA Kit | AE59290HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: BACE1

Alternative Name: ASP2; BACE; FLJ90568; HSPC104; KIAA1149; APP beta-secretase|aspartyl protease 2|beta-secretase|beta-site amyloid beta A4 precursor protein-cleaving enzyme|beta-site amyloid precursor protein cleavin

Application: ELISA

Range: 0.250-16 ng/mL

Sensitivity: 0.11 ng/mL

Intra-Assay: ≤5.2%

Inter-Assay: ≤8.9%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate BACE1 in samples. An antibody specific for BACE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBACE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BACE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BACE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: β-Secretaseis an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein, contains two active site aspartate residues in its extracellular protein domain and may function as a dimer.Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the amyloid precursor protein (APP) . Extracellular cleavage of APP by BACE releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by γ-secretase. The second cleavage releases the intracellular domain of APP and amyloid-β. Since alpha-secretase cleaves APP closer to the cell membrane than BACE does.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .