Human BIN1 (Bridging Integrator 1) ELISA Kit | ELK4191

Human BIN1 (Bridging Integrator 1) ELISA Kit | ELK4191 | Gentaur UK, US & Europe Distribution

Alternative Names: AMPH2; AMPHL; SH3P9; Amphiphysin II; Myc Box-Dependent-Interacting Protein 1; Amphiphysin-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bridging Integrator 1 (BIN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bridging Integrator 1 (BIN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bridging Integrator 1 (BIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bridging Integrator 1 (BIN1) in the samples is then determined by comparing the OD of the samples to the standard curve.