Human BMP10 (Bone Morphogenetic Protein 10) ELISA Kit | ELK3193

Human BMP10 (Bone Morphogenetic Protein 10) ELISA Kit | ELK3193 | Gentaur UK, US & Europe Distribution

Alternative Names: BMP-10; Bmp10; BMP10

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cardiovascular biology;Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 10 (BMP10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 10 (BMP10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 10 (BMP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 10 (BMP10) in the samples is then determined by comparing the OD of the samples to the standard curve.