Human BNP (Brain Natriuretic Peptide) ELISA Kit | ELK4892

Human BNP (Brain Natriuretic Peptide) ELISA Kit | ELK4892 | Gentaur UK, US & Europe Distribution

Alternative Names: GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 9.67 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Cardiovascular biology;Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Brain Natriuretic Peptide (BNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Brain Natriuretic Peptide (BNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Natriuretic Peptide (BNP) in the samples is then determined by comparing the OD of the samples to the standard curve.