Human BTLA (B- and T-lymphocyte attenuator) ELISA Kit | ELK8669

Human BTLA (B- and T-lymphocyte attenuator) ELISA Kit | ELK8669 | Gentaur UK, US & Europe Distribution

Alternative Names: B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; BTLA1; CD272; CD272 antigen; FLJ16065; MGC129743

Species: Human

Assay Type: Sandwich

Sensitivity: 0.22 ng/mL

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: Serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cardiac function; Cancer

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B- and T-lymphocyte attenuator (BTLA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B- and T-lymphocyte attenuator (BTLA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B- and T-lymphocyte attenuator (BTLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B- and T-lymphocyte attenuator (BTLA) in the samples is then determined by comparing the OD of the samples to the standard curve.