Human C-Myc-binding protein (MYCBP) ELISA Kit | KTE62538

Human C-Myc-binding protein (MYCBP) ELISA Kit | KTE62538 | Gentaur UK, US & Europe Distribution

Application: This Human C-Myc-binding protein (MYCBP) ELISA Kit employs a two-site sandwich ELISA to quantitate MYCBP in samples. An antibody specific for MYCBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYCBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYCBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYCBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human C-Myc-binding protein microplate
• Human C-Myc-binding protein standard
• Human C-Myc-binding protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human C-Myc-binding protein (MYCBP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYCBP. No significant cross-reactivity or interference between Human MYCBP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The MYCBP gene encodes a protein that binds to the N-terminal region of MYC and stimulates the activation of E box-dependent transcription by MYC.The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis. A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cellular factors. AMY1 was found to bind in vitro and in vivo to the regulatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84. AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggested that AMY1 plays a role in spermatogenesis.

Alternative Names: MYCBP; AMY-1; FLJ41056; associate of myc-1

Search name: MYCBP; AMY-1; FLJ41056; associate of myc-1

Tag: MYCBP