Human C5a (Complement Component 5a) ELISA Kit | ELK1051

Human C5a (Complement Component 5a) ELISA Kit | ELK1051 | Gentaur UK, US & Europe Distribution

Alternative Names: Complement Component 5a

Species: Human

Assay Type: Sandwich

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a (C5a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 5a (C5a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5a (C5a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5a (C5a) in the samples is then determined by comparing the OD of the samples to the standard curve.