Human C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit | ELK3025

Human C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit | ELK3025 | Gentaur UK, US & Europe Distribution

Alternative Names: MAC; Membrane Attack Complex

Species: Human

Assay Type: Sandwich

Sensitivity: 0.51 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Terminal Complement Complex C5b-9 (C5b-9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Terminal Complement Complex C5b-9 (C5b-9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Terminal Complement Complex C5b-9 (C5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Terminal Complement Complex C5b-9 (C5b-9) in the samples is then determined by comparing the OD of the samples to the standard curve.