Human CA1 (Carbonic Anhydrase I) ELISA Kit | ELK1432

Human CA1 (Carbonic Anhydrase I) ELISA Kit | ELK1432 | Gentaur UK, US & Europe Distribution

Alternative Names: CA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

Species: Human

Assay Type: Sandwich

Sensitivity: 0.65 pg/mL

Standard: 100 pg/mL

Detection Range: 1.57-100 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase I (CA1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbonic Anhydrase I (CA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase I (CA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase I (CA1) in the samples is then determined by comparing the OD of the samples to the standard curve.