Human CA125 (Carbohydrate Antigen 125) ELISA Kit | ELK2322

Human CA125 (Carbohydrate Antigen 125) ELISA Kit | ELK2322 | Gentaur UK, US & Europe Distribution

Alternative Names: MUC16; Cancer Antigen 125; Mucin 16, Cell Surface Associated; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 U/mL

Standard: 100 U/mL

Detection Range: 1.57-100 U/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 125 (CA125) in the samples is then determined by comparing the OD of the samples to the standard curve.