Human CA19-5 (Carbohydrate Antigen 19-5) ELISA Kit | ELK7082

Human CA19-5 (Carbohydrate Antigen 19-5) ELISA Kit | ELK7082 | Gentaur UK, US & Europe Distribution

Alternative Names: CA19-5

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.86 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Tumor immunity;Hepatology;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carbohydrate Antigen 19-5 (CA19-5) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-5 (CA19-5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 19-5 (CA19-5) in the samples is then determined by comparing the OD of the samples to the standard curve.