Human CA9 (Carbonic Anhydrase IX) ELISA Kit | ELK1373

Human CA9 (Carbonic Anhydrase IX) ELISA Kit | ELK1373 | Gentaur UK, US & Europe Distribution

Alternative Names: CA-IX; CAIX; MN; pMW1; P54/58N; Carbonic Dehydratase; Renal cell carcinoma-associated antigen G250; RCC-Associated Protein G250; Carbonate dehydratase IX

Species: Human

Assay Type: Sandwich

Sensitivity: 2.9 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase IX (CA9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbonic Anhydrase IX (CA9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IX (CA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase IX (CA9) in the samples is then determined by comparing the OD of the samples to the standard curve.