Human CACNa1D (Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit | ELK3707

Human CACNa1D (Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit | ELK3707 | Gentaur UK, US & Europe Distribution

Alternative Names: CACH3; CACN4; CACNL1A2; CCHL1A2; Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3; Calcium channel, L type, alpha-1 polypeptide, isoform 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) in the samples is then determined by comparing the OD of the samples to the standard curve.