Human CACNg6 (Calcium Channel, Voltage Dependent, Gamma Subunit 6) ELISA Kit | ELK7727

Human CACNg6 (Calcium Channel, Voltage Dependent, Gamma Subunit 6) ELISA Kit | ELK7727 | Gentaur UK, US & Europe Distribution

Alternative Names: Neuronal voltage-gated calcium channel gamma-6 subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, Gamma Subunit 6 (CACNg6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, Gamma Subunit 6 (CACNg6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, Gamma Subunit 6 (CACNg6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, Gamma Subunit 6 (CACNg6) in the samples is then determined by comparing the OD of the samples to the standard curve.