Human Canalicular multispecific organic anion transporter 2 (ABCC3) ELISA Kit | KTE60860

Human Canalicular multispecific organic anion transporter 2 (ABCC3) ELISA Kit | KTE60860 | Gentaur UK, US & Europe Distribution

Application: This Human Canalicular multispecific organic anion transporter 2 (ABCC3) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC3 in samples. An antibody specific for ABCC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Canalicular multispecific organic anion transporter 2 microplate
• Human Canalicular multispecific organic anion transporter 2 standard
• Human Canalicular multispecific organic anion transporter 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Canalicular multispecific organic anion transporter 2 (ABCC3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCC3. No significant cross-reactivity or interference between Human ABCC3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Canalicular multispecific organic anion transporter 2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) . This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Alternative Names: ABCC3; ABC31; DKFZp686E22157; EST90757; MLP2; MOAT-D; MRP3; cMOAT2; ATP-binding cassette; sub-family C; member 3; canicular multispecific organic anion transporter; multidrug resistance associated protein

Search name: ABCC3; ABC31; DKFZp686E22157; EST90757; MLP2; MOAT-D; MRP3; cMOAT2; ATP-binding cassette; sub-family C; member 3; canicular multispecific organic anion transporter; multidrug resistance associated protein

Tag: ABCC3