Human carboxyhemoglobin, HbCO ELISA Kit | ELK8401

Human carboxyhemoglobin, HbCO ELISA Kit | ELK8401 | Gentaur UK, US & Europe Distribution

Alternative Names: HbCO

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.36 ng/mL

Standard: 2000 ng/mL

Detection Range: 31.25-2000 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Cardiovascular biology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with carboxyhemoglobin, HbCO protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to carboxyhemoglobin, HbCO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of carboxyhemoglobin, HbCO in the samples is then determined by comparing the OD of the samples to the standard curve.