Human Cathepsin B (CTSB) ELISA Kit | KTE62688

Human Cathepsin B (CTSB) ELISA Kit | KTE62688 | Gentaur UK, US & Europe Distribution

Application: This Human Cathepsin B (CTSB) ELISA Kit employs a two-site sandwich ELISA to quantitate CTSB in samples. An antibody specific for CTSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cathepsin B microplate
• Human Cathepsin B standard
• Human Cathepsin B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cathepsin B (CTSB) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTSB. No significant cross-reactivity or interference between Human CTSB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. The protein is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of β-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade β-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.

Alternative Names: CTSB; APPS; CPSB; APP secretase; amyloid precursor protein secretase; cathepsin B1; cysteine protease; preprocathepsin B

Search name: CTSB; APPS; CPSB; APP secretase; amyloid precursor protein secretase; cathepsin B1; cysteine protease; preprocathepsin B

Tag: CTSB