Human CBG (Corticosteroid Binding Globulin) ELISA Kit | ELK3040

Human CBG (Corticosteroid Binding Globulin) ELISA Kit | ELK3040 | Gentaur UK, US & Europe Distribution

Alternative Names: SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor, Clade A, Member 6 (Alpha-1 Antiproteinase; Antitrypsin)

Species: Human

Assay Type: Sandwich

Sensitivity: 0.073 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticosteroid Binding Globulin (CBG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticosteroid Binding Globulin (CBG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticosteroid Binding Globulin (CBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticosteroid Binding Globulin (CBG) in the samples is then determined by comparing the OD of the samples to the standard curve.