Human CD20 protein, His tag (Animal-Free) | PRP2025

Human CD20 protein, His tag (Animal-Free) | PRP2025 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from human CD20 (NP_068769.2) (Glu213-Pro297) was expressed with a 6 His tag at the C-terminus.

Activity: N/A

protein Lenght: The recombinant human CD20 protein consists of 91 amino acids and predicts a molecular mass of 10.6 KD. It migrates as an approximately 23 KD band in SDS-PAGE under reducing conditions due to glycosylation.

Preparation: HEK293 Cells

Purity: >90% as determined by SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 10.6 KD

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD20 protein alpha protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human CD20 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.101% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells.  CD20/MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20/MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) . CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Alternative Names: CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Search Names: CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Tag: CD20